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EXECUTIVE SUMMARY Live Oak Associates, Inc. conducted an investigation of the biological resources of an approximately 23-acre parcel located in Pleasanton, Alameda County, California to assess possible regulatory constraints to future site development. The study site is located south of Mohr Avenue, just south of a large water retention basin, and north of a truck yard that is north of Busch Road within the City of Pleasanton, Alameda County, Califomia. The proposed project, Ironwood 55+, is an active community and development will consist of 110 detached, single-family homes and a private recreational facility for residents. Currently, the site consists of a ruderal landscape and is used for borrow material. The site itself does not support any natural water features, and therefore, development of the site will not fall under the jurisdiction of the U.S. Army Corp of Engineers (USAGE), the California Department of Fish and Game (CDFG), or the Regional Water Quality Control Board (RWQCB) for regulated habitats. The overall biological value of the site has been low for several decades as it had been an intensely farm for several decades and more recently converted to a site that consist primarily of ruderal vegetation. As such, this habitat provides low quality habitat for wildlife and thus, tends to be inhabitated by common species adapted to living in urban disturbed settings. The site does support a small population of California ground squirrels, and therefore also supports potential habitat for the burrowing owl, California species of special concern. However, no burrowing owls were observed during reconnaissance surveys conducted in February and April 2008 (during the breeding season), and there aze only five recorded observation of burrowing owls within three miles of the site, all recorded in 1993. Though no evidence of bunrowing owls (e.g., white wash, pellets, feathers) was observed onsite during reconnaissance level surveys, potentially suitable habitat for the owl is present in the small number of ground squirrel burrows on site. Therefore, pre-construction protocol-level surveys would need to be conducted by a qualified biologist for the burrowing owl to determine if they remain absence from the site. Due to the fact no owls have been observed within three miles of the site since 1993, it is unlikely burrowing owls would be found onsite. However, if burrowing owls were found onsite in the future, and prior to site development, the owls would need to be avoided if found during the breeding season, or evicted if found during the non-breeding season to avoid causing nest failure or harming individuals. Live Oak Associates, /nc.