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Environmental Checklist and City of Pleasanton–10x Genomics Project <br />Environmental Evaluation Initial Study/Mitigated Negative Declaration <br /> <br /> <br />52 FirstCarbon Solutions <br />Https://adecinnovations.sharepoint.com/sites/PublicationsSite/Shared Documents/Publications/Client (PN-JN)/2148/21480024/ISMND/wp/21480024 10x Genomics Project Full Screencheck ISMND.docx <br />c) Have a substantial adverse effect on State or federally protected wetlands (including, but not <br />limited to, marsh, vernal pool, coastal, etc.) through direct removal, filling, hydrological <br />interruption, or other means? <br />No Impact. Prior to conducting the field survey of the project site, FCS reviewed existing topographic <br />maps, aerial photography as well as the online resources including the United States Fish and <br />Wildlife Service (USFWS), National Wetlands Inventory 31 to determine if any potentially <br />jurisdictional water bodies or wetlands exist within the vicinity of the project site. The project site is <br />entirely developed and does not contain any potential jurisdictional waters or wetlands. Therefore, <br />the proposed project would not have a substantially adverse effect on State or federally protected <br />wetlands. No impact would occur. <br />d) Interfere substantially with the movement of any native resident or migratory fish or wildlife <br />species or with established native resident or migratory wildlife corridors, or impede the use of <br />wildlife nursery sites? <br />No Impact. The project site is located within an urban and built-up area of Pleasanton. The project <br />site contains impervious surfaces, lacks native vegetation, and is surrounded by urban development <br />including commercial businesses and busy roadways in all directions. These structures serve as <br />significant barriers to wildlife movement through the project site and vicinity. As such, the proposed <br />project would not interfere substantially with the movement of wildlife. No impact would occur. <br />e) Conflict with any local policies or ordinances protecting biological resources, such as a tree <br />preservation policy or ordinance? <br />Less than significant with mitigation incorporated. Prior to conducting the field survey of the <br />project site, FCS reviewed the Pleasanton General Plan for relevant local policies or ordinances <br />related to the protection and preservation of biological resources. FCS also reviewed a Preliminary <br />Arborist Report (Appendix B) prepared for the project site in 2021.32 The plan identified 159 trees <br />on-site, of which 70 are classified as Heritage Trees, based on their species and/or size as described <br />below. <br />The Pleasanton General Plan was adopted in 2009 and includes policies on Heritage Tree <br />preservation. Heritage trees are illegal to remove without the appropriate permit. Chapter 17.16 of <br />the Municipal Code defines a Heritage Tree as: <br />3. Any single-trunked tree with a circumference of 55 inches or more measured four and one <br />half feet above ground level; <br />4. Any multi-trunked tree of which the two largest trunks have a circumference of 55 inches or <br />more measured four and one-half feet above ground level; <br />5. Any tree 35 feet or more in height; <br /> <br />31 United States Fish and Wildlife Service (USFWS). 2020. https://www.fws.gov/wetlands/data/mapper.html Accessed December 14, <br />2020. <br />32 Hort Science. 2021. Preliminary Arborist Report 1701 Springdale Avenue, Pleasanton, CA.